Dextran-grafted polycation copolymer to enhance DNAzyme activity and refine DNAzyme-based biosensing
نویسندگان
چکیده
منابع مشابه
Design and biosensing of Mg²⁺-dependent DNAzyme-triggered ratiometric electrochemiluminescence.
A dual-potential ratiometric electrochemiluminescence (ECL) sensing approach based on Mg(2+)-dependent DNAzyme-regulated ECL signals of luminol and CdS quantum dots (QDs) is designed. The system consists of DNAzyme strand functionalized QDs as capture probes and cathode ECL emitters, luminol-reduced gold nanoparticles (Au@luminol) as anode ECL emitters, and a Mg(2+) substrate strand modified wi...
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10-23 DNAzyme has the potential to suppress gene expressions through sequence-specific mRNA cleavage. However, the dependence on exogenous delivery limits its applications. The objective of this work is to establish a replicating DNAzyme in bacteria using a single-stranded DNA vector. By cloning the 10-23 DNAzyme into the M13mp18 vector, we constructed two circular DNAzymes, C-Dz7 and C-Dz482, ...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2016
ISSN: 2296-4185
DOI: 10.3389/conf.fbioe.2016.01.01054